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rabbit polyclonal anti phospho eif2α ser51  (Biorbyt)
93
Biorbyt rabbit polyclonal anti phospho eif2α ser51
Regorafenib reduces ZIKV E protein, but not NS1, levels and does not activate the integrated stress response. ( A ) A549 cells were either left untreated (Untr) or were treated with DMSO (vehicle control), Axitinib (Ax; 10 µM), Dabrafenib (Db; 10 µM), or Regorafenib (Rg; 2.5 µM) for the indicated durations. As a positive control, cells were treated with DTT (2 mM) for 30 min prior to harvesting. Whole-cell lysates were analyzed by Western blotting with antibodies against phospho-eIF2α <t>(Ser51),</t> total eIF2α, and vinculin (loading control). ( B ) A549 cells were mock-infected or infected with ZIKV (MOI = 3 PFU/cell) and were left untreated (Untr) or were treated with DMSO (vehicle control), Axitinib (Ax; 10 µM), Dabrafenib (Db; 10 µM), or Regorafenib (Rg; 2.5 µM) for the indicated durations. As a positive control, cells were treated with DTT (2 mM) for 30 min prior to harvesting. Whole-cell lysates were analyzed by Western blotting with antibodies against phospho-eIF2α (Ser51), total eIF2α, ZIKV E protein, and vinculin (loading control). Ratios of phospho-eIF2α to total eIF2α (P/T) and the relative levels of ZIKV E protein and ZIKV NS1 were determined by densitometric analysis. The latter are shown as bar graphs and are expressed as mean ± standard deviation (SD). **, P < 0.01; ****, P < 0.0001 (multiple t test with Holm–Šidák correction vs “DMSO”). Blots in ( A ) and ( B ) are representative of two independent experiments. ( C ) A549 cells were treated with DTT (2 mM) for 30 min prior to fixation and SGs were visualized by immunofluorescence staining for G3BP1 (red). Nuclei were stained with Hoechst 33342 (blue). Scale bar, 50 µm. ( D ) A549 cells were mock-infected or infected with ZIKV (MOI = 3 PFU/cell) and were left untreated (Untr) or treated with DMSO (vehicle control) or Regorafenib (2.5 µM) for 24 h. ZIKV infection and SGs were visualized by immunofluorescence staining for ZIKV E protein (green) and G3BP1 (red), respectively. Scale bars, 50 µm. Images are representative of two independent experiments.
Rabbit Polyclonal Anti Phospho Eif2α Ser51, supplied by Biorbyt, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit polyclonal anti-phospho-eukaryotic initiation factor 2 [anti-phospho-eif2 α ] antibody  (Cell Signaling Technology Inc)
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Regorafenib reduces ZIKV E protein, but not NS1, levels and does not activate the integrated stress response. ( A ) A549 cells were either left untreated (Untr) or were treated with DMSO (vehicle control), Axitinib (Ax; 10 µM), Dabrafenib (Db; 10 µM), or Regorafenib (Rg; 2.5 µM) for the indicated durations. As a positive control, cells were treated with DTT (2 mM) for 30 min prior to harvesting. Whole-cell lysates were analyzed by Western blotting with antibodies against phospho-eIF2α <t>(Ser51),</t> total eIF2α, and vinculin (loading control). ( B ) A549 cells were mock-infected or infected with ZIKV (MOI = 3 PFU/cell) and were left untreated (Untr) or were treated with DMSO (vehicle control), Axitinib (Ax; 10 µM), Dabrafenib (Db; 10 µM), or Regorafenib (Rg; 2.5 µM) for the indicated durations. As a positive control, cells were treated with DTT (2 mM) for 30 min prior to harvesting. Whole-cell lysates were analyzed by Western blotting with antibodies against phospho-eIF2α (Ser51), total eIF2α, ZIKV E protein, and vinculin (loading control). Ratios of phospho-eIF2α to total eIF2α (P/T) and the relative levels of ZIKV E protein and ZIKV NS1 were determined by densitometric analysis. The latter are shown as bar graphs and are expressed as mean ± standard deviation (SD). **, P < 0.01; ****, P < 0.0001 (multiple t test with Holm–Šidák correction vs “DMSO”). Blots in ( A ) and ( B ) are representative of two independent experiments. ( C ) A549 cells were treated with DTT (2 mM) for 30 min prior to fixation and SGs were visualized by immunofluorescence staining for G3BP1 (red). Nuclei were stained with Hoechst 33342 (blue). Scale bar, 50 µm. ( D ) A549 cells were mock-infected or infected with ZIKV (MOI = 3 PFU/cell) and were left untreated (Untr) or treated with DMSO (vehicle control) or Regorafenib (2.5 µM) for 24 h. ZIKV infection and SGs were visualized by immunofluorescence staining for ZIKV E protein (green) and G3BP1 (red), respectively. Scale bars, 50 µm. Images are representative of two independent experiments.
Rabbit Polyclonal Anti Phospho Eukaryotic Initiation Factor 2 [Anti Phospho Eif2 α ] Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit polyclonal anti-phospho-eukaryotic initiation factor 2 [anti-phospho-eif2 α ] antibody - by Bioz Stars, 2026-09
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rabbit polyclonal anti p eif2  (Cell Signaling Technology Inc)
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Regorafenib reduces ZIKV E protein, but not NS1, levels and does not activate the integrated stress response. ( A ) A549 cells were either left untreated (Untr) or were treated with DMSO (vehicle control), Axitinib (Ax; 10 µM), Dabrafenib (Db; 10 µM), or Regorafenib (Rg; 2.5 µM) for the indicated durations. As a positive control, cells were treated with DTT (2 mM) for 30 min prior to harvesting. Whole-cell lysates were analyzed by Western blotting with antibodies against phospho-eIF2α <t>(Ser51),</t> total eIF2α, and vinculin (loading control). ( B ) A549 cells were mock-infected or infected with ZIKV (MOI = 3 PFU/cell) and were left untreated (Untr) or were treated with DMSO (vehicle control), Axitinib (Ax; 10 µM), Dabrafenib (Db; 10 µM), or Regorafenib (Rg; 2.5 µM) for the indicated durations. As a positive control, cells were treated with DTT (2 mM) for 30 min prior to harvesting. Whole-cell lysates were analyzed by Western blotting with antibodies against phospho-eIF2α (Ser51), total eIF2α, ZIKV E protein, and vinculin (loading control). Ratios of phospho-eIF2α to total eIF2α (P/T) and the relative levels of ZIKV E protein and ZIKV NS1 were determined by densitometric analysis. The latter are shown as bar graphs and are expressed as mean ± standard deviation (SD). **, P < 0.01; ****, P < 0.0001 (multiple t test with Holm–Šidák correction vs “DMSO”). Blots in ( A ) and ( B ) are representative of two independent experiments. ( C ) A549 cells were treated with DTT (2 mM) for 30 min prior to fixation and SGs were visualized by immunofluorescence staining for G3BP1 (red). Nuclei were stained with Hoechst 33342 (blue). Scale bar, 50 µm. ( D ) A549 cells were mock-infected or infected with ZIKV (MOI = 3 PFU/cell) and were left untreated (Untr) or treated with DMSO (vehicle control) or Regorafenib (2.5 µM) for 24 h. ZIKV infection and SGs were visualized by immunofluorescence staining for ZIKV E protein (green) and G3BP1 (red), respectively. Scale bars, 50 µm. Images are representative of two independent experiments.
Rabbit Polyclonal Anti P Eif2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rabbit polyclonal anti p eif2  (Cell Signaling Technology Inc)
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Regorafenib reduces ZIKV E protein, but not NS1, levels and does not activate the integrated stress response. ( A ) A549 cells were either left untreated (Untr) or were treated with DMSO (vehicle control), Axitinib (Ax; 10 µM), Dabrafenib (Db; 10 µM), or Regorafenib (Rg; 2.5 µM) for the indicated durations. As a positive control, cells were treated with DTT (2 mM) for 30 min prior to harvesting. Whole-cell lysates were analyzed by Western blotting with antibodies against phospho-eIF2α <t>(Ser51),</t> total eIF2α, and vinculin (loading control). ( B ) A549 cells were mock-infected or infected with ZIKV (MOI = 3 PFU/cell) and were left untreated (Untr) or were treated with DMSO (vehicle control), Axitinib (Ax; 10 µM), Dabrafenib (Db; 10 µM), or Regorafenib (Rg; 2.5 µM) for the indicated durations. As a positive control, cells were treated with DTT (2 mM) for 30 min prior to harvesting. Whole-cell lysates were analyzed by Western blotting with antibodies against phospho-eIF2α (Ser51), total eIF2α, ZIKV E protein, and vinculin (loading control). Ratios of phospho-eIF2α to total eIF2α (P/T) and the relative levels of ZIKV E protein and ZIKV NS1 were determined by densitometric analysis. The latter are shown as bar graphs and are expressed as mean ± standard deviation (SD). **, P < 0.01; ****, P < 0.0001 (multiple t test with Holm–Šidák correction vs “DMSO”). Blots in ( A ) and ( B ) are representative of two independent experiments. ( C ) A549 cells were treated with DTT (2 mM) for 30 min prior to fixation and SGs were visualized by immunofluorescence staining for G3BP1 (red). Nuclei were stained with Hoechst 33342 (blue). Scale bar, 50 µm. ( D ) A549 cells were mock-infected or infected with ZIKV (MOI = 3 PFU/cell) and were left untreated (Untr) or treated with DMSO (vehicle control) or Regorafenib (2.5 µM) for 24 h. ZIKV infection and SGs were visualized by immunofluorescence staining for ZIKV E protein (green) and G3BP1 (red), respectively. Scale bars, 50 µm. Images are representative of two independent experiments.
Rabbit Polyclonal Anti P Eif2, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rabbit+polyclonal+anti-phospho-eif2+antibody/Phospho-eIF2alpha+(Ser51)+Antibody/10__1074_slash_jbc__ra118__002121-191-117-120
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rabbit polyclonal anti p eif2 - by Bioz Stars, 2026-09
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rabbit anti phospho eif2 ser 51 polyclonal antibody  (Cell Signaling Technology Inc)
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Regorafenib reduces ZIKV E protein, but not NS1, levels and does not activate the integrated stress response. ( A ) A549 cells were either left untreated (Untr) or were treated with DMSO (vehicle control), Axitinib (Ax; 10 µM), Dabrafenib (Db; 10 µM), or Regorafenib (Rg; 2.5 µM) for the indicated durations. As a positive control, cells were treated with DTT (2 mM) for 30 min prior to harvesting. Whole-cell lysates were analyzed by Western blotting with antibodies against phospho-eIF2α <t>(Ser51),</t> total eIF2α, and vinculin (loading control). ( B ) A549 cells were mock-infected or infected with ZIKV (MOI = 3 PFU/cell) and were left untreated (Untr) or were treated with DMSO (vehicle control), Axitinib (Ax; 10 µM), Dabrafenib (Db; 10 µM), or Regorafenib (Rg; 2.5 µM) for the indicated durations. As a positive control, cells were treated with DTT (2 mM) for 30 min prior to harvesting. Whole-cell lysates were analyzed by Western blotting with antibodies against phospho-eIF2α (Ser51), total eIF2α, ZIKV E protein, and vinculin (loading control). Ratios of phospho-eIF2α to total eIF2α (P/T) and the relative levels of ZIKV E protein and ZIKV NS1 were determined by densitometric analysis. The latter are shown as bar graphs and are expressed as mean ± standard deviation (SD). **, P < 0.01; ****, P < 0.0001 (multiple t test with Holm–Šidák correction vs “DMSO”). Blots in ( A ) and ( B ) are representative of two independent experiments. ( C ) A549 cells were treated with DTT (2 mM) for 30 min prior to fixation and SGs were visualized by immunofluorescence staining for G3BP1 (red). Nuclei were stained with Hoechst 33342 (blue). Scale bar, 50 µm. ( D ) A549 cells were mock-infected or infected with ZIKV (MOI = 3 PFU/cell) and were left untreated (Untr) or treated with DMSO (vehicle control) or Regorafenib (2.5 µM) for 24 h. ZIKV infection and SGs were visualized by immunofluorescence staining for ZIKV E protein (green) and G3BP1 (red), respectively. Scale bars, 50 µm. Images are representative of two independent experiments.
Rabbit Anti Phospho Eif2 Ser 51 Polyclonal Antibody, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Regorafenib reduces ZIKV E protein, but not NS1, levels and does not activate the integrated stress response. ( A ) A549 cells were either left untreated (Untr) or were treated with DMSO (vehicle control), Axitinib (Ax; 10 µM), Dabrafenib (Db; 10 µM), or Regorafenib (Rg; 2.5 µM) for the indicated durations. As a positive control, cells were treated with DTT (2 mM) for 30 min prior to harvesting. Whole-cell lysates were analyzed by Western blotting with antibodies against phospho-eIF2α <t>(Ser51),</t> total eIF2α, and vinculin (loading control). ( B ) A549 cells were mock-infected or infected with ZIKV (MOI = 3 PFU/cell) and were left untreated (Untr) or were treated with DMSO (vehicle control), Axitinib (Ax; 10 µM), Dabrafenib (Db; 10 µM), or Regorafenib (Rg; 2.5 µM) for the indicated durations. As a positive control, cells were treated with DTT (2 mM) for 30 min prior to harvesting. Whole-cell lysates were analyzed by Western blotting with antibodies against phospho-eIF2α (Ser51), total eIF2α, ZIKV E protein, and vinculin (loading control). Ratios of phospho-eIF2α to total eIF2α (P/T) and the relative levels of ZIKV E protein and ZIKV NS1 were determined by densitometric analysis. The latter are shown as bar graphs and are expressed as mean ± standard deviation (SD). **, P < 0.01; ****, P < 0.0001 (multiple t test with Holm–Šidák correction vs “DMSO”). Blots in ( A ) and ( B ) are representative of two independent experiments. ( C ) A549 cells were treated with DTT (2 mM) for 30 min prior to fixation and SGs were visualized by immunofluorescence staining for G3BP1 (red). Nuclei were stained with Hoechst 33342 (blue). Scale bar, 50 µm. ( D ) A549 cells were mock-infected or infected with ZIKV (MOI = 3 PFU/cell) and were left untreated (Untr) or treated with DMSO (vehicle control) or Regorafenib (2.5 µM) for 24 h. ZIKV infection and SGs were visualized by immunofluorescence staining for ZIKV E protein (green) and G3BP1 (red), respectively. Scale bars, 50 µm. Images are representative of two independent experiments.
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rabbit anti p eif2 α polyclonal antibody  (Cell Signaling Technology Inc)
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Regorafenib reduces ZIKV E protein, but not NS1, levels and does not activate the integrated stress response. ( A ) A549 cells were either left untreated (Untr) or were treated with DMSO (vehicle control), Axitinib (Ax; 10 µM), Dabrafenib (Db; 10 µM), or Regorafenib (Rg; 2.5 µM) for the indicated durations. As a positive control, cells were treated with DTT (2 mM) for 30 min prior to harvesting. Whole-cell lysates were analyzed by Western blotting with antibodies against phospho-eIF2α <t>(Ser51),</t> total eIF2α, and vinculin (loading control). ( B ) A549 cells were mock-infected or infected with ZIKV (MOI = 3 PFU/cell) and were left untreated (Untr) or were treated with DMSO (vehicle control), Axitinib (Ax; 10 µM), Dabrafenib (Db; 10 µM), or Regorafenib (Rg; 2.5 µM) for the indicated durations. As a positive control, cells were treated with DTT (2 mM) for 30 min prior to harvesting. Whole-cell lysates were analyzed by Western blotting with antibodies against phospho-eIF2α (Ser51), total eIF2α, ZIKV E protein, and vinculin (loading control). Ratios of phospho-eIF2α to total eIF2α (P/T) and the relative levels of ZIKV E protein and ZIKV NS1 were determined by densitometric analysis. The latter are shown as bar graphs and are expressed as mean ± standard deviation (SD). **, P < 0.01; ****, P < 0.0001 (multiple t test with Holm–Šidák correction vs “DMSO”). Blots in ( A ) and ( B ) are representative of two independent experiments. ( C ) A549 cells were treated with DTT (2 mM) for 30 min prior to fixation and SGs were visualized by immunofluorescence staining for G3BP1 (red). Nuclei were stained with Hoechst 33342 (blue). Scale bar, 50 µm. ( D ) A549 cells were mock-infected or infected with ZIKV (MOI = 3 PFU/cell) and were left untreated (Untr) or treated with DMSO (vehicle control) or Regorafenib (2.5 µM) for 24 h. ZIKV infection and SGs were visualized by immunofluorescence staining for ZIKV E protein (green) and G3BP1 (red), respectively. Scale bars, 50 µm. Images are representative of two independent experiments.
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Regorafenib reduces ZIKV E protein, but not NS1, levels and does not activate the integrated stress response. ( A ) A549 cells were either left untreated (Untr) or were treated with DMSO (vehicle control), Axitinib (Ax; 10 µM), Dabrafenib (Db; 10 µM), or Regorafenib (Rg; 2.5 µM) for the indicated durations. As a positive control, cells were treated with DTT (2 mM) for 30 min prior to harvesting. Whole-cell lysates were analyzed by Western blotting with antibodies against phospho-eIF2α (Ser51), total eIF2α, and vinculin (loading control). ( B ) A549 cells were mock-infected or infected with ZIKV (MOI = 3 PFU/cell) and were left untreated (Untr) or were treated with DMSO (vehicle control), Axitinib (Ax; 10 µM), Dabrafenib (Db; 10 µM), or Regorafenib (Rg; 2.5 µM) for the indicated durations. As a positive control, cells were treated with DTT (2 mM) for 30 min prior to harvesting. Whole-cell lysates were analyzed by Western blotting with antibodies against phospho-eIF2α (Ser51), total eIF2α, ZIKV E protein, and vinculin (loading control). Ratios of phospho-eIF2α to total eIF2α (P/T) and the relative levels of ZIKV E protein and ZIKV NS1 were determined by densitometric analysis. The latter are shown as bar graphs and are expressed as mean ± standard deviation (SD). **, P < 0.01; ****, P < 0.0001 (multiple t test with Holm–Šidák correction vs “DMSO”). Blots in ( A ) and ( B ) are representative of two independent experiments. ( C ) A549 cells were treated with DTT (2 mM) for 30 min prior to fixation and SGs were visualized by immunofluorescence staining for G3BP1 (red). Nuclei were stained with Hoechst 33342 (blue). Scale bar, 50 µm. ( D ) A549 cells were mock-infected or infected with ZIKV (MOI = 3 PFU/cell) and were left untreated (Untr) or treated with DMSO (vehicle control) or Regorafenib (2.5 µM) for 24 h. ZIKV infection and SGs were visualized by immunofluorescence staining for ZIKV E protein (green) and G3BP1 (red), respectively. Scale bars, 50 µm. Images are representative of two independent experiments.

Journal: Journal of Virology

Article Title: The Raf kinase inhibitors Dabrafenib and Regorafenib impair Zika virus replication via distinct mechanisms

doi: 10.1128/jvi.00618-24

Figure Lengend Snippet: Regorafenib reduces ZIKV E protein, but not NS1, levels and does not activate the integrated stress response. ( A ) A549 cells were either left untreated (Untr) or were treated with DMSO (vehicle control), Axitinib (Ax; 10 µM), Dabrafenib (Db; 10 µM), or Regorafenib (Rg; 2.5 µM) for the indicated durations. As a positive control, cells were treated with DTT (2 mM) for 30 min prior to harvesting. Whole-cell lysates were analyzed by Western blotting with antibodies against phospho-eIF2α (Ser51), total eIF2α, and vinculin (loading control). ( B ) A549 cells were mock-infected or infected with ZIKV (MOI = 3 PFU/cell) and were left untreated (Untr) or were treated with DMSO (vehicle control), Axitinib (Ax; 10 µM), Dabrafenib (Db; 10 µM), or Regorafenib (Rg; 2.5 µM) for the indicated durations. As a positive control, cells were treated with DTT (2 mM) for 30 min prior to harvesting. Whole-cell lysates were analyzed by Western blotting with antibodies against phospho-eIF2α (Ser51), total eIF2α, ZIKV E protein, and vinculin (loading control). Ratios of phospho-eIF2α to total eIF2α (P/T) and the relative levels of ZIKV E protein and ZIKV NS1 were determined by densitometric analysis. The latter are shown as bar graphs and are expressed as mean ± standard deviation (SD). **, P < 0.01; ****, P < 0.0001 (multiple t test with Holm–Šidák correction vs “DMSO”). Blots in ( A ) and ( B ) are representative of two independent experiments. ( C ) A549 cells were treated with DTT (2 mM) for 30 min prior to fixation and SGs were visualized by immunofluorescence staining for G3BP1 (red). Nuclei were stained with Hoechst 33342 (blue). Scale bar, 50 µm. ( D ) A549 cells were mock-infected or infected with ZIKV (MOI = 3 PFU/cell) and were left untreated (Untr) or treated with DMSO (vehicle control) or Regorafenib (2.5 µM) for 24 h. ZIKV infection and SGs were visualized by immunofluorescence staining for ZIKV E protein (green) and G3BP1 (red), respectively. Scale bars, 50 µm. Images are representative of two independent experiments.

Article Snippet: The following primary antibodies were used: mouse monoclonal anti-ZIKV NS1 antibody (1:1,000; clone GT5212, GeneTex), rabbit polyclonal anti-ZIKV E protein antibody (1:1,000; Biorbyt), rabbit polyclonal anti-phospho-eIF2α (Ser51) antibody (1:2,000; Proteintech), rabbit polyclonal anti-eIF2α antibody (1:2,000; Proteintech), rabbit monoclonal anti-GAPDH antibody (1:2,000; clone D16H11, Cell Signaling Technology), and rabbit monoclonal anti-vinculin antibody (1:5,000; clone 3M13, Sigma-Aldrich).

Techniques: Control, Positive Control, Western Blot, Infection, Standard Deviation, Immunofluorescence, Staining